Early Phase Bioprospecting and Phenotypic Characterization of Streptomycetes in Greater Accra

HIV in Sunyani and peri-urban areas

  • Reuben Essel Arhin Department of Science Laboratory Technology, Accra Technical University, Ghana & Department of Medical Microbiology, College of Health Sciences, University of Ghana Medical School, Ghana
  • Diana Ahu Prah Department of Science Laboratory Technology, Accra Technical University, Ghana & West Africa Centre for Cell Biology of Infectious Pathogens, University of Ghana, Ghana
  • Lawal Samad Department of Science Laboratory Technology, Accra Technical University, Ghana
  • Ben Nbronbin Boyour Department of Science Laboratory Technology, Accra Technical University, Ghana
  • Edmund Ofosu Kwabi Department of Science Laboratory Technology, Accra Technical University, Ghana
  • Kelvin Essuman Department of Science Laboratory Technology, Accra Technical University, Ghana
  • Andrew Adjei Ahlidza Department of Science Laboratory Technology, Accra Technical University, Ghana
  • Priscilla Delasi Hamenu Department of Science Laboratory Technology, Accra Technical University, Ghana
  • Nathaniel Asare Department of Science Laboratory Technology, Accra Technical University, Ghana
  • Kingsley Nii Ayittey Nalai Boye Department of Science Laboratory Technology, Accra Technical University, Ghana
  • Victoria Sowah Department of Science Laboratory Technology, Accra Technical University, Ghana
  • Mohammed Alim Department of Science Laboratory Technology, Accra Technical University, Ghana
  • Rayyan Ahmed Yahaya Department of Science Laboratory Technology, Accra Technical University, Ghana
  • Frederick Shelvin Agyei Department of Science Laboratory Technology, Accra Technical University, Ghana
  • Rosemary Mawuse Afreh Accra Technical University
  • Samuel Owiredu Department of Science Laboratory Technology, Accra Technical University, Ghana
DOI: https://doi.org/10.46829/hsijournal.2024.7.6.1.783-789
Keywords: Bioprospecting, antimicrobial, phenotypic, streptomycetes, Ghana

Abstract

Introduction: Microbial life forms including streptomycetes are an important source of natural products obtained from bioprospecting. The aim of this study was to provide baseline data on the phenotypic diversity of streptomycetes from soils in Greater Accra and to determine the suitability of the selected areas for bioprospecting of potentially novel antimicrobial-producing strains.

Methodology: Twenty-one soil samples were collected from 7 areas in Greater Accra. Streptomycetes were cultured on Oatmeal Agar for sporulation. Spores were subcultured onto a variety of media to ascertain the colony morphology of the strains. Extracts of pure isolates were obtained via submerged cultures. The antimicrobial activity of the extracts was determined against clinical bacteria using the agar well diffusion method and categorized by their antimicrobial inhibition halo diameter.

Results: A total of 15 phenotypically proven diverse strains of streptomycetes were recovered from the soil samples. Two of the isolates were antimicrobial producers. Tryptone Soya Broth extract of Streptomyces sp. V1 showed good efficacy (++) against Pseudomonas aeruginosa (22 ± 2 mm) whereas Streptomyces sp. W2 showed good efficacy (++) against Escherichia coli (20 ± 8 mm) and moderate efficacy (+) against Pseudomonas aeruginosa (13 ± 2 mm).  

Conclusions: Soils from different areas in Ghana may be potential sources of the next novel antimicrobial producing streptomycetes.

Published
2024-07-30
Issue
Vol 6 No 1 (2024): Health Sciences Investigations Journal
Section
Original Research Article