CYP2B6 and SULT1A1 single nucleotide polymorphism  among Ghanaian HIV patients :

Isaac Egyir Prah; Isaac Julius Asiedu-Gyekye; Edmund Tetteh Nartey; Elvis Twumasi Aboagye; William Kudzi

doi:10.46829/hsijournal.2024.12.6.2.983-989

Isaac Egyir Prah Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, Accra, Ghana
Isaac Julius Asiedu-Gyekye Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, Accra, Ghana
Edmund Tetteh Nartey Centre for Tropical Clinical Pharmacology and Therapeutics, University of Ghana Medical School, College of Health Sciences, University of Ghana, Accra, Ghana
Elvis Twumasi Aboagye Centre for Tropical Clinical Pharmacology and Therapeutics, University of Ghana Medical School, College of Health Sciences, University of Ghana, Accra, Ghana
William Kudzi Centre for Tropical Clinical Pharmacology and Therapeutics, University of Ghana Medical School, College of Health Sciences, University of Ghana, Accra, Ghana

DOI: https://doi.org/10.46829/hsijournal.2024.12.6.2.983-989

Keywords: CYP2B6, HIV, Nevirapine, SULT1A1

Abstract

Background: Nevirapine is used in many developing countries for the management of HIV-1 patients. Despite its usefulness, hypersensitivity reaction is a common complication that accounts for patients defaulting during therapy in Ghana. Genetic variations in drug-metabolising enzymes have been implicated in reported adverse drug reactions observed in patients on nevirapine regimen.
Objective: This study aimed to determine genotypic frequencies of specific CYP2B6 and SULT1A1 variants and their association with nevirapine hypersensitivity among persons living with HIV in the Ghanaian population.
Methods: A prospective study was conducted in Korle-Bu Teaching Hospital, a tertiary health facility in Ghana. Clinical data were recorded from the seventy patients’ folder, and whole blood was collected for genotyping. Genotypes of CYP2B6c.983T > C and SULT1A1c.638G > A were obtained using Restriction Fragment Length Polymorphism method.
Results: The mean age of the participants was 38 ± 9.47 years, with the majority 77% being females. For CYP2B6c.983T > C genotype frequencies, T/T and T/C were 94.3% and 5.7%, respectively, while for SULT1A1c.638G > A genotype frequencies, G/G, G/A, and A/A were 61.4%, 34.3% and 4.3% respectively. The prevalence of CYP2B6c.983T > C and SULT1A1c.638G > A minor allele was 2.9% and 21.4%, respectively among the study participants.
Conclusion: Extensive metaboliser genotypes for CYP2B6c.983T > C and SULT1A1c.638G > A were more than the intermediate and poor metaboliser genotype. However, CYP2B6 983C/C representing poor metabolisers of CYP2B6c.983T>C were not detected among the study population. Genetic polymorphism of CYP2B6c.983T > C and SULT1A1c.638G > A were not associated with nevirapine hypersensitivity.