Assessment of DNA Quality of Chrysophyllum perpulcrum (Mildbr.ex Hutch. & Dalziel) and Chrysophyllum albidum (G.Don) using CTAB-Based Method

Abstract

The establishment of the cetyltrimethyl ammonium bromide (CTAB) protocol in plants has been very useful in DNA extraction. This has been useful in the extraction of high-quality DNA from plants for molecular studies. This study was carried out to assess the quality (in terms of purity, concentration, and yield) of the DNA extracted from the leaves of Chrysophyllum perpulcrum and Chrysophyllum albidum using the CTAB-based method. A modified CTAB extraction protocol was used for the extraction of DNA from the leaves sampled from mature trees of Chrysophyllum perpulcrum, Chrysophyllum albidum and wildlings of Chrysophyllum perpulcrum. Although this study revealed that a significant difference (P > 0.05) did not exist in the DNA purity (at an absorbance ratio of A260/280), DNA concentration, or yield of DNA, Chrysophyllum perpulcrum wildlings had the highest DNA yield (7.43 µg) and DNA concentration (742.96 µg/ml). The DNA purity for this study was in this order: 1.82125 (Ca) < 1.87750 (Cpm) < 1.87800 (Cpw). A ratio of about 1.8 is generally accepted as ‘‘pure’’ for DNA. This implies that the DNA isolated using the CTAB-based method is suitable for further molecular analysis and, as such, is recommended.